freestyletm 293 expression medium (Thermo Fisher)
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freestyletm 293 expression medium
Freestyletm 293 Expression Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/freestyletm+293+expression+medium/freestyletm+293+expression+medium/pmc10268256__pnas__2219404120__sapp-7-34-40
Average 90 stars, based on 1 article reviews
Freestyletm 293 Expression Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/freestyletm+293+expression+medium/freestyletm+293+expression+medium/pmc10268256__pnas__2219404120__sapp-7-34-40
Average 90 stars, based on 1 article reviews
freestyletm 293 expression medium - by Bioz Stars,
2026-09
90/100 stars
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Suspension:Article Title: An optimizing lentiviral titer determination assay based on Raji cells Article Snippet: .. For suspension culture of 293T cells, the cells were adapted to grow in suspension by gradual acclimatization to Article Title: Gender difference in pre-clinical liver-directed gene therapy with lentiviral vectors Article Snippet: The human embryonic kidney cell line expressing the SV40 large T antigen (HEK293T) was obtained from ATCC (LGC Standards, Teddington, United Kingdom) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Merck Life Science, Dorset, United Kingdom) supplemented with 10% heat-inactivated fetal bovine serum (FBS; ThermoFisher Scientific, United Kingdom), 2 mM L-glutamine (Merck Life Science) and 1% non-essential amino acids (NEAAs; Merck Life Science). .. HEK293T cells adapted to culture in suspension phase were maintained in Article Title: Cryo-EM structures of mouse bestrophin 1 channel in closed and partially open conformations. Article Snippet: Plasmid construct, cell culture, and transfection The full coding sequence of mouse bestrophin-1 (NM_011913.2) was cloned into the EEV vector (System Biosciences, Palo Alto, CA, United States), with a 3C protease cleavage site fused in-frame to the BRIL-3xFLAG tag coding sequence at the 3’end. .. HEK293-GnTI- cells were cultured in a suspension system at 37°C under 5% humidified CO2 with shaking at 150 rpm, using Expressing:Article Title: An optimizing lentiviral titer determination assay based on Raji cells Article Snippet: .. For suspension culture of 293T cells, the cells were adapted to grow in suspension by gradual acclimatization to Article Title: Gender difference in pre-clinical liver-directed gene therapy with lentiviral vectors Article Snippet: The human embryonic kidney cell line expressing the SV40 large T antigen (HEK293T) was obtained from ATCC (LGC Standards, Teddington, United Kingdom) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Merck Life Science, Dorset, United Kingdom) supplemented with 10% heat-inactivated fetal bovine serum (FBS; ThermoFisher Scientific, United Kingdom), 2 mM L-glutamine (Merck Life Science) and 1% non-essential amino acids (NEAAs; Merck Life Science). .. HEK293T cells adapted to culture in suspension phase were maintained in Article Title: Cryo-EM structures of mouse bestrophin 1 channel in closed and partially open conformations. Article Snippet: Plasmid construct, cell culture, and transfection The full coding sequence of mouse bestrophin-1 (NM_011913.2) was cloned into the EEV vector (System Biosciences, Palo Alto, CA, United States), with a 3C protease cleavage site fused in-frame to the BRIL-3xFLAG tag coding sequence at the 3’end. .. HEK293-GnTI- cells were cultured in a suspension system at 37°C under 5% humidified CO2 with shaking at 150 rpm, using Article Title: Supporting Information for NgR1 binding to reovirus reveals an unusual bivalent interaction and a new viral attachment protein Article Snippet: Lec2 and Lec2-NgR1 cells were maintained in MEM α nucleosides medium (Gibco) supplemented to contain 10% FBS, 100 units/mL penicillin, 100 μg/mL streptomycin, and 2 mM L-glutamine. .. CHO cells (ATCC, CCL-61) were maintained in Ham’s F12 medium supplemented to contain 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 0.25 mg/mL amphotericin B. FreestyleTM 293-F cells (ThermoFisher Scientific) were maintained in Article Title: Potentiating anti-tumor immunity by re-engaging immune synapse molecules Article Snippet: McCoy’s 5A Medium (Modified) (1X) , Thermo Fisher Scientific , Cat# 16600082. .. Article Title: Gating mechanism of the two-pore-domain potassium channel THIK1. Article Snippet: TWIK-related halothane-inhibited potassium channel (THIK1) maintains the resting membrane potential and regulates potassium efflux in microglia.. It is a potential therapeutic target for neurodegenerative disorders, neuropathic pain and inflammation.. However, the mechanism underlying its function remains unclear. Article Title: Modular cell line for scalable and rapid in vitro evaluation of chimeric antigen receptors. Article Snippet: Plasmid DNA was purified using the PureLinkTM HiPure Plasmid Miniprep Kit (#K210003, Thermo Fisher, USA) for eukaryotic expression in the FreeStyleTM 293 cell line. .. A total of 30 × 10^6 cells in 30 ml of Article Title: Structural basis of human cytomegalovirus neutralization by gB AD-5-specific potent antibodies. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE-Cy5 Mouse Anti-Human CD16 BD Biosciences Cat# 555408; RRID: AB_395808 PE Mouse Anti-Human IgD BD Biosciences Cat# 555779; RRID: AB_396114 FITC Mouse Anti-Human CD14 BD Biosciences Cat# 555397; RRID: AB_395798 PE-Cy5 Mouse Anti-Human CD3 BD Biosciences Cat# 555341; RRID: AB_395747 APC-H7 Mouse Anti-Human CD19 BD Biosciences Cat# 560177; RRID: AB_1645470 PE Mouse Anti-Human CD235a BD Biosciences Cat# 555570; RRID: AB_395949 PE Goat Anti-Human IgG Thermo Fisher Scientific Cat# 12-4998-82; RRID: AB_465926 PE Goat Anti-Rabbit IgG Thermo Fisher Scientific Cat# P-2771MP; RRID: AB_2539845 Goat Anti-Human IgG HRP Promega Cat# W4031; RRID: AB_430835 Goat Anti-Human IgG FITC Solarbio Cat# SF101 Goat Anti-Mouse IgG FITC Solarbio Cat# SF131 Goat Anti-Mouse IgG-HRP Promega Cat# W4021; RRID: AB_430834 Goat Anti-Human IgG-HRP Promega Cat# W4301 Rabbit Anti-Human CD64 Abcam Cat# ab196571 PE Goat Anti-Rabbit IgG SouthernBiotech Cat# 4030-09; RRID: AB_2795937 PE-Cy5 Mouse Anti-human IgG BD Biosciences Cat# 551497; RRID: AB_394220 8F9 Thomson et al.38 mAb produced in this study SM5-1 Spindler et al.35 mAb produced in this study 1G2 Chandramouli et al.36 mAb produced in this study SM10 Pötzsch et al.17 mAb produced in this study MSL-109 Fouts et al.68 mAb produced in this study Mouse Anti-CMV gH Santa Cruz Biotechnology Cat# sc-58113 Mouse Anti-cytomegalovirus IE1 and IE2 Abcam Cat# ab53495; RRID: AB_882995 Cells and virus strains HEK293T/17 ATCC Cat# CRL-11268; RRID:CVCL_1926 Expi293F Cells Thermo Fisher Scientific Cat# A14527 HEK293S GnTI− Cells Thermo Fisher Scientific N/A MRC-5 Cells ATCC Cat# CCL-171; RRID:CVCL_0440 ARPE-19 ATCC Cat# CRL-2302; RRID:CVCL_0145 CHO cells Horizon Discovery NA gB-stabilized CHO cells This paper NA HCMV Towne strain Rutgers University, USA N/A HCMV TB40/E strain Pasteur Institute, China N/A HCMV BE13/2012 strain Anhui Medical University N/A VR1814 Rutgers University, USA N/A NR Rutgers University, USA N/A Chemicals and proteins 0.25% Trypsin-EDTA(1X) Gibco Cat# 25200-072 Dulbecco’s Modified Eagle Medium Gibco Cat# C11885500BT 2xDMEM GENOM, China Cat# GNM12902-2 Fetal bovine serum (FBS) Gibco Cat# 16000-044 Penicillin-streptomycin Gibco Cat# Incubation:Article Title: An optimizing lentiviral titer determination assay based on Raji cells Article Snippet: .. For suspension culture of 293T cells, the cells were adapted to grow in suspension by gradual acclimatization to Cell Culture:Article Title: Cryo-EM structures of mouse bestrophin 1 channel in closed and partially open conformations. Article Snippet: Plasmid construct, cell culture, and transfection The full coding sequence of mouse bestrophin-1 (NM_011913.2) was cloned into the EEV vector (System Biosciences, Palo Alto, CA, United States), with a 3C protease cleavage site fused in-frame to the BRIL-3xFLAG tag coding sequence at the 3’end. .. HEK293-GnTI- cells were cultured in a suspension system at 37°C under 5% humidified CO2 with shaking at 150 rpm, using Article Title: Gating mechanism of the two-pore-domain potassium channel THIK1. Article Snippet: TWIK-related halothane-inhibited potassium channel (THIK1) maintains the resting membrane potential and regulates potassium efflux in microglia.. It is a potential therapeutic target for neurodegenerative disorders, neuropathic pain and inflammation.. However, the mechanism underlying its function remains unclear. Molecular Cloning:Article Title: Gating mechanism of the two-pore-domain potassium channel THIK1. Article Snippet: TWIK-related halothane-inhibited potassium channel (THIK1) maintains the resting membrane potential and regulates potassium efflux in microglia.. It is a potential therapeutic target for neurodegenerative disorders, neuropathic pain and inflammation.. However, the mechanism underlying its function remains unclear. Transfection:Article Title: Modular cell line for scalable and rapid in vitro evaluation of chimeric antigen receptors. Article Snippet: Plasmid DNA was purified using the PureLinkTM HiPure Plasmid Miniprep Kit (#K210003, Thermo Fisher, USA) for eukaryotic expression in the FreeStyleTM 293 cell line. .. A total of 30 × 10^6 cells in 30 ml of Produced:Article Title: Structural basis of human cytomegalovirus neutralization by gB AD-5-specific potent antibodies. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE-Cy5 Mouse Anti-Human CD16 BD Biosciences Cat# 555408; RRID: AB_395808 PE Mouse Anti-Human IgD BD Biosciences Cat# 555779; RRID: AB_396114 FITC Mouse Anti-Human CD14 BD Biosciences Cat# 555397; RRID: AB_395798 PE-Cy5 Mouse Anti-Human CD3 BD Biosciences Cat# 555341; RRID: AB_395747 APC-H7 Mouse Anti-Human CD19 BD Biosciences Cat# 560177; RRID: AB_1645470 PE Mouse Anti-Human CD235a BD Biosciences Cat# 555570; RRID: AB_395949 PE Goat Anti-Human IgG Thermo Fisher Scientific Cat# 12-4998-82; RRID: AB_465926 PE Goat Anti-Rabbit IgG Thermo Fisher Scientific Cat# P-2771MP; RRID: AB_2539845 Goat Anti-Human IgG HRP Promega Cat# W4031; RRID: AB_430835 Goat Anti-Human IgG FITC Solarbio Cat# SF101 Goat Anti-Mouse IgG FITC Solarbio Cat# SF131 Goat Anti-Mouse IgG-HRP Promega Cat# W4021; RRID: AB_430834 Goat Anti-Human IgG-HRP Promega Cat# W4301 Rabbit Anti-Human CD64 Abcam Cat# ab196571 PE Goat Anti-Rabbit IgG SouthernBiotech Cat# 4030-09; RRID: AB_2795937 PE-Cy5 Mouse Anti-human IgG BD Biosciences Cat# 551497; RRID: AB_394220 8F9 Thomson et al.38 mAb produced in this study SM5-1 Spindler et al.35 mAb produced in this study 1G2 Chandramouli et al.36 mAb produced in this study SM10 Pötzsch et al.17 mAb produced in this study MSL-109 Fouts et al.68 mAb produced in this study Mouse Anti-CMV gH Santa Cruz Biotechnology Cat# sc-58113 Mouse Anti-cytomegalovirus IE1 and IE2 Abcam Cat# ab53495; RRID: AB_882995 Cells and virus strains HEK293T/17 ATCC Cat# CRL-11268; RRID:CVCL_1926 Expi293F Cells Thermo Fisher Scientific Cat# A14527 HEK293S GnTI− Cells Thermo Fisher Scientific N/A MRC-5 Cells ATCC Cat# CCL-171; RRID:CVCL_0440 ARPE-19 ATCC Cat# CRL-2302; RRID:CVCL_0145 CHO cells Horizon Discovery NA gB-stabilized CHO cells This paper NA HCMV Towne strain Rutgers University, USA N/A HCMV TB40/E strain Pasteur Institute, China N/A HCMV BE13/2012 strain Anhui Medical University N/A VR1814 Rutgers University, USA N/A NR Rutgers University, USA N/A Chemicals and proteins 0.25% Trypsin-EDTA(1X) Gibco Cat# 25200-072 Dulbecco’s Modified Eagle Medium Gibco Cat# C11885500BT 2xDMEM GENOM, China Cat# GNM12902-2 Fetal bovine serum (FBS) Gibco Cat# 16000-044 Penicillin-streptomycin Gibco Cat# Virus:Article Title: Structural basis of human cytomegalovirus neutralization by gB AD-5-specific potent antibodies. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE-Cy5 Mouse Anti-Human CD16 BD Biosciences Cat# 555408; RRID: AB_395808 PE Mouse Anti-Human IgD BD Biosciences Cat# 555779; RRID: AB_396114 FITC Mouse Anti-Human CD14 BD Biosciences Cat# 555397; RRID: AB_395798 PE-Cy5 Mouse Anti-Human CD3 BD Biosciences Cat# 555341; RRID: AB_395747 APC-H7 Mouse Anti-Human CD19 BD Biosciences Cat# 560177; RRID: AB_1645470 PE Mouse Anti-Human CD235a BD Biosciences Cat# 555570; RRID: AB_395949 PE Goat Anti-Human IgG Thermo Fisher Scientific Cat# 12-4998-82; RRID: AB_465926 PE Goat Anti-Rabbit IgG Thermo Fisher Scientific Cat# P-2771MP; RRID: AB_2539845 Goat Anti-Human IgG HRP Promega Cat# W4031; RRID: AB_430835 Goat Anti-Human IgG FITC Solarbio Cat# SF101 Goat Anti-Mouse IgG FITC Solarbio Cat# SF131 Goat Anti-Mouse IgG-HRP Promega Cat# W4021; RRID: AB_430834 Goat Anti-Human IgG-HRP Promega Cat# W4301 Rabbit Anti-Human CD64 Abcam Cat# ab196571 PE Goat Anti-Rabbit IgG SouthernBiotech Cat# 4030-09; RRID: AB_2795937 PE-Cy5 Mouse Anti-human IgG BD Biosciences Cat# 551497; RRID: AB_394220 8F9 Thomson et al.38 mAb produced in this study SM5-1 Spindler et al.35 mAb produced in this study 1G2 Chandramouli et al.36 mAb produced in this study SM10 Pötzsch et al.17 mAb produced in this study MSL-109 Fouts et al.68 mAb produced in this study Mouse Anti-CMV gH Santa Cruz Biotechnology Cat# sc-58113 Mouse Anti-cytomegalovirus IE1 and IE2 Abcam Cat# ab53495; RRID: AB_882995 Cells and virus strains HEK293T/17 ATCC Cat# CRL-11268; RRID:CVCL_1926 Expi293F Cells Thermo Fisher Scientific Cat# A14527 HEK293S GnTI− Cells Thermo Fisher Scientific N/A MRC-5 Cells ATCC Cat# CCL-171; RRID:CVCL_0440 ARPE-19 ATCC Cat# CRL-2302; RRID:CVCL_0145 CHO cells Horizon Discovery NA gB-stabilized CHO cells This paper NA HCMV Towne strain Rutgers University, USA N/A HCMV TB40/E strain Pasteur Institute, China N/A HCMV BE13/2012 strain Anhui Medical University N/A VR1814 Rutgers University, USA N/A NR Rutgers University, USA N/A Chemicals and proteins 0.25% Trypsin-EDTA(1X) Gibco Cat# 25200-072 Dulbecco’s Modified Eagle Medium Gibco Cat# C11885500BT 2xDMEM GENOM, China Cat# GNM12902-2 Fetal bovine serum (FBS) Gibco Cat# 16000-044 Penicillin-streptomycin Gibco Cat# Modification:Article Title: Structural basis of human cytomegalovirus neutralization by gB AD-5-specific potent antibodies. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PE-Cy5 Mouse Anti-Human CD16 BD Biosciences Cat# 555408; RRID: AB_395808 PE Mouse Anti-Human IgD BD Biosciences Cat# 555779; RRID: AB_396114 FITC Mouse Anti-Human CD14 BD Biosciences Cat# 555397; RRID: AB_395798 PE-Cy5 Mouse Anti-Human CD3 BD Biosciences Cat# 555341; RRID: AB_395747 APC-H7 Mouse Anti-Human CD19 BD Biosciences Cat# 560177; RRID: AB_1645470 PE Mouse Anti-Human CD235a BD Biosciences Cat# 555570; RRID: AB_395949 PE Goat Anti-Human IgG Thermo Fisher Scientific Cat# 12-4998-82; RRID: AB_465926 PE Goat Anti-Rabbit IgG Thermo Fisher Scientific Cat# P-2771MP; RRID: AB_2539845 Goat Anti-Human IgG HRP Promega Cat# W4031; RRID: AB_430835 Goat Anti-Human IgG FITC Solarbio Cat# SF101 Goat Anti-Mouse IgG FITC Solarbio Cat# SF131 Goat Anti-Mouse IgG-HRP Promega Cat# W4021; RRID: AB_430834 Goat Anti-Human IgG-HRP Promega Cat# W4301 Rabbit Anti-Human CD64 Abcam Cat# ab196571 PE Goat Anti-Rabbit IgG SouthernBiotech Cat# 4030-09; RRID: AB_2795937 PE-Cy5 Mouse Anti-human IgG BD Biosciences Cat# 551497; RRID: AB_394220 8F9 Thomson et al.38 mAb produced in this study SM5-1 Spindler et al.35 mAb produced in this study 1G2 Chandramouli et al.36 mAb produced in this study SM10 Pötzsch et al.17 mAb produced in this study MSL-109 Fouts et al.68 mAb produced in this study Mouse Anti-CMV gH Santa Cruz Biotechnology Cat# sc-58113 Mouse Anti-cytomegalovirus IE1 and IE2 Abcam Cat# ab53495; RRID: AB_882995 Cells and virus strains HEK293T/17 ATCC Cat# CRL-11268; RRID:CVCL_1926 Expi293F Cells Thermo Fisher Scientific Cat# A14527 HEK293S GnTI− Cells Thermo Fisher Scientific N/A MRC-5 Cells ATCC Cat# CCL-171; RRID:CVCL_0440 ARPE-19 ATCC Cat# CRL-2302; RRID:CVCL_0145 CHO cells Horizon Discovery NA gB-stabilized CHO cells This paper NA HCMV Towne strain Rutgers University, USA N/A HCMV TB40/E strain Pasteur Institute, China N/A HCMV BE13/2012 strain Anhui Medical University N/A VR1814 Rutgers University, USA N/A NR Rutgers University, USA N/A Chemicals and proteins 0.25% Trypsin-EDTA(1X) Gibco Cat# 25200-072 Dulbecco’s Modified Eagle Medium Gibco Cat# C11885500BT 2xDMEM GENOM, China Cat# GNM12902-2 Fetal bovine serum (FBS) Gibco Cat# 16000-044 Penicillin-streptomycin Gibco Cat# |